Urinary tract infections (UTIs) are considered the most widespread bacterial infections and among the most common nosocomial infections, second only to lower respiratory tract infections. The risk of developing a UTI increases with age, making UTIs among the most commonly diagnosed infections in elderly people, representing a significant public health problem. This study aimed to isolate and detect the microbial responsible for urinary tract infections in elderly individuals and determine their resistance patterns and sensitivity to antibiotics among Libyan patients in Tripoli city. The study was conducted simultaneously in Alsharik, Eltafoq, and Ibn Al Nafis laboratories in Tripoli, Libya, during July and August 2024. One hundred and one urine samples were collected from elderly participants suffering from UTI symptoms (38 males and 63 females), with ages ranging from 60 to 97 years. Isolation, biochemical identification, and antibiotic susceptibility analyses were performed. Thirteen antibiotics were testedin this study. Among the 101 isolated samples from elderly patients with suspected UTI, females had a higher prevalence of bacterial infections (62.4%) compared to males (37.6%). The highest number of infections was in the 60–69 years age group (45.4%). Gram-negative organisms accounted for 91.1% of isolates compared to Gram-positive organisms. Escherichia coli was the most common organism isolated (43.6%), followed by Klebsiella pneumoniae (29.7%). Most isolates were resistant to Ampicillin (31.7%) and Amoxicillin (23.8%), followed by Tetracycline (10.9%). The Gram-positive organisms Streptococcus pneumoniae, Enterobacter spp., and Staphylococcus aureus were most susceptible to Amoxicillin, Amikacin, and Meropenem, with sensitivity rates of 75%, 66.7%, and50%, respectively. E. coli was more susceptible to Imipenem (25%), Amikacin (22.7%), and Nitrofurantoin (13.6%), whereas Amoxicillin and Sulfamethoxazole/Trimethoprim showed lower efficacy. Gram-negative bacteria were the main cause of UTIs in the study population, mainly belonging to the E. coli and Klebsiella families. Age and gender were significant factors in determining UTI etiology, and considering these factors can improve the accuracy in identifying causative uropathogens and guide empirical treatment.Keywords:UTI, Antibiotic Resistance, Sensitivity Antibiotics, Uropathogenic E.Coli

Acute conjunctivitis is described as inflammation of the conjunctiva that lasts less than 3 to 4 weeks, showing cellular infiltration and exudation. Conjunctivitis can cause significant and lasting harm to the eye, resulting in severe inflammation in the corneal lid. This study seeks to isolate and identify the particular bacterial pathogens responsible for conjunctivitis in patients at Tripoli Eye Hospital. Collection of bacterial samples from patients with conjunctivitis was conducted at the ophthalmic consultative clinic within Tripoli Eye Hospital, Libya between September 2021 and January 2022. Tests were done to separate bacterial pathogens causing conjunctivitis and to test their resistance to antibiotics. Among 140 samples, bacterial infection was detected in 86% of cases while 14% yielded negative results. Males had a higher prevalence of bacterial infections at 63% in comparison to females. Both male and female individuals aged 60-79 years had the highest percentage of bacterial conjunctivitis cases at 51%. Segregation tests showed that the majority (82%) were Gram-positive, while a minority (18%) were Gram-negative, with Staphylococcus aureus being the most prevalent bacteria (35%), closely followed by Staphylococcus epidermidis (32%). Streptococcal pneumoniae and Enterobacter spp had rates of 2% which were lower. The majority of organisms showed sensitivity to levofloxacin (43%), while there was only a small amount of resistance to polymyxin B (4%). Professional attention and microbiological research are crucial for verifying clinical diagnoses and carrying out suitable and efficient treatments.